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To receive news and publication updates for Oxidative Medicine and Cellular Longevity, enter your email address in the box below. A corrigendum for this article has been published. To view the corrigendum, please click here. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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An ideal method for the analysis of N -glycans would both identify the isomeric structure and deliver a true picture of the relative, if not absolute, amounts of the various structures in one sample. Porous graphitic carbon chromatography coupled with electrospray ionization mass spectrometry ESI-MS detection has emerged as a method with a particularly high potential of resolving isomeric oligosaccharides, but little attention has so far been paid to quantitation of the results obtained. When subjected to liquid chromatography—ESI-MS in positive and negative modes, the glyco tune mix clearly demonstrated the futility of quantitation of N -glycans of different overall composition, different number of sialic acids, and strongly differing size without compensation for their very different molar responses.

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Insertion of the insertion sequence Is2 I directly before the galE gene of the galactose operon results in a Gal minus phenotype 1, 2. No promoters intrinsic to the bp of the IS2 II sequence also present on the template were detected.

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